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fuw dcas9 tet1cd p2a bfp  (Addgene inc)


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    Structured Review

    Addgene inc fuw dcas9 tet1cd p2a bfp
    Fuw Dcas9 Tet1cd P2a Bfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fuw+dcas9+plasmid/Fuw-dCas9-Tet1CD-P2A-BFP+(Plasmid+%23108245)/pm41896915-81-8-14
    Average 92 stars, based on 8 article reviews
    fuw dcas9 tet1cd p2a bfp - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells
    Article Snippet: The PCR program was as follows: 5 min at 95 °C for predenaturation, followed by 35 cycles of 95 °C for 15 s, 60 °C for 1 min, and 72 °C for 15 s. The PCR products were subjected to 1% agarose gel electrophoresis to separate them, and bands of the desired sizes were excised, purified, and ligated with the pEAZY-T1 vector for Sanger sequencing. .. The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively. .. Subsequently, we used T4 Ligase (TAKARA) to form the final circular fuw-dCas9- dnmt3a plasmid.

    Plasmid Preparation:

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells
    Article Snippet: The PCR program was as follows: 5 min at 95 °C for predenaturation, followed by 35 cycles of 95 °C for 15 s, 60 °C for 1 min, and 72 °C for 15 s. The PCR products were subjected to 1% agarose gel electrophoresis to separate them, and bands of the desired sizes were excised, purified, and ligated with the pEAZY-T1 vector for Sanger sequencing. .. The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively. .. Subsequently, we used T4 Ligase (TAKARA) to form the final circular fuw-dCas9- dnmt3a plasmid.

    Article Title: Epigenetic editing of Indian Medaka fgf2 using CRISPR/dCas9-tet1CD
    Article Snippet: .. The fuw-dCas9 plasmid (Addgene #84476) (Liu 615 et al., 2016) was digested with HamI (NEB) and EcoRI (NEB) at 37°C for 2 hours. .. The 616 digested plasmid and the PCR-amplified tet1 product were subsequently subjected to 617 homologous recombination using the ligase (Vazyme, C116-02, China) to generate a 618 circular fuw-dCas9-tet1 plasmid.



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